Table 2.
Fatty acid composition of total lipids from different human brain regions comparing middle-aged and elderly individuals. Healthy individuals were grouped as middle-aged (< 60 years) and elderly (> 60 years). Differences between groups were assessed by applying a t-test and corrected by FDR method of Benjamini and Hochberg, with Q = 10%. Values are reported in mol% as mean ± SEM from 8 to 9 individuals × group. P < 0.05 was selected as the minimum level of statistical significance. Significance compared to middle-aged group prior FDR correction is denoted using asterisks (*P < 0.05; **P < 0.01; ***P < 0.001)
HINDBRAIN | MIDBRAIN | ||||||||||||||||||||||
Olive | Vermis | Substantia nigra | |||||||||||||||||||||
Middle-aged | Elderly | Sig | Middle-aged | Elderly | Sig | Middle-aged | Elderly | Sig | |||||||||||||||
14:0 | 1.84 ± 0.19 | 1.29 ± 0.12* | 0.546 | 1.08 ± 0.05 | 0.99 ± 0.05 | 0.803 | 0.61 ± 0.02 | 0.65 ± 0.02 | 0.578 | ||||||||||||||
16:0 | 15.36 ± 0.26 | 15.78 ± 0.44 | 0.775 | 24.5 ± 0.56 | 24.7 ± 0.38 | 0.908 | 17.55 ± 0.18 | 18.28 ± 0.49 | 0.457 | ||||||||||||||
16:1n7 | 2.23 ± 0.09 | 2.11 ± 0.25 | 0.775 | 1.22 ± 0.08 | 1.14 ± 0.07 | 0.803 | 1.22 ± 0.03 | 1.35 ± 0.05* | 0.229 | ||||||||||||||
18:0 | 20.11 ± 0.52 | 19.24 ± 0.81 | 0.597 | 20.28 ± 0.18 | 20.82 ± 0.48 | 0.803 | 22.57 ± 0.13 | 22.98 ± 0.29 | 0.571 | ||||||||||||||
18:1n9 | 30.57 ± 0.35 | 31.3 ± 2.48 | 0.597 | 20.81 ± 0.56 | 20.44 ± 0.84 | 0.813 | 24.1 ± 0.33 | 24.06 ± 1.00 | 0.947 | ||||||||||||||
18:1n7 | 5.19 ± 0.16 | 5.28 ± 0.17 | 0.917 | 4.37 ± 0.16 | 4.15 ± 0.13 | 0.803 | 5.08 ± 0.09 | 5.18 ± 0.19 | 0.805 | ||||||||||||||
18:2n6 | 0.62 ± 0.11 | 0.54 ± 0.09 | 0.775 | 0.94 ± 0.11 | 0.92 ± 0.07 | 0.944 | 0.73 ± 0.07 | 0.71 ± 0.061 | 0.879 | ||||||||||||||
18:3n3 | 0.18 ± 0.00 | 0.17 ± 0.01 | 0.775 | 0.07 ± 0.00 | 0.07 ± 0.00 | 0.803 | 0.11 ± 0.00 | 0.1 ± 0.00 | 0.571 | ||||||||||||||
18:4n3 | 0.05 ± 0.00 | 0.03 ± 0.00 | 0.597 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.803 | 0.02 ± 0.00 | 0.04 ± 0.00* | 0.173 | ||||||||||||||
20:0 | 0.44 ± 0.09 | 0.38 ± 0.03 | 0.775 | 0.23 ± 0.00 | 0.28 ± 0.01** | 0.068 | 0.24 ± 0.01 | 0.24 ± 0.00 | 0.947 | ||||||||||||||
20:1n9 | 4.54 ± 0.21 | 4.17 ± 0.68 | 0.775 | 1.40 ± 0.11 | 1.41 ± 0.14 | 0.946 | 2.31 ± 0.23 | 2.04 ± 0.15 | 0.578 | ||||||||||||||
20:2n6 | 0.86 ± 0.08 | 0.95 ± 0.12 | 0.774 | 0.29 ± 0.03 | 0.26 ± 0.01 | 0.803 | 0.45 ± 0.03 | 0.41 ± 0.04 | 0.658 | ||||||||||||||
20:3n6 | 1.53 ± 0.10 | 1.38 ± 0.11 | 0.753 | 1.02 ± 0.07 | 0.9 ± 0.06 | 0.803 | 1.42 ± 0.06 | 1.28 ± 0.12 | 0.571 | ||||||||||||||
20:4n6 | 4.3 ± 0.14 | 4.77 ± 0.73 | 0.565 | 8.02 ± 0.15 | 7.82 ± 0.28 | 0.803 | 6.95 ± 0.22 | 7.2 ± 0.23 | 0.607 | ||||||||||||||
20:5n3 | 0.05 ± 0.00 | 0.05 ± 0.01 | 0.981 | 0.05 ± 0.01 | 0.03 ± 0.01 | 0.803 | 0.14 ± 0.01 | 0.12 ± 0.01 | 0.571 | ||||||||||||||
22:0 | 0.4 ± 0.12 | 0.31 ± 0.04 | 0.775 | 0.07 ± 0.00 | 0.07 ± 0.00 | 0.944 | 0.13 ± 0.01 | 0.11 ± 0.01 | 0.578 | ||||||||||||||
22:1n9 | 0.83 ± 0.10 | 0.73 ± 0.79 | 0.775 | 0.85 ± 0.05 | 0.93 ± 0.06 | 0.803 | 0.96 ± 0.16 | 0.8 ± 0.34 | 0.616 | ||||||||||||||
22:4n6 | 3.78 ± 0.17 | 4.00 ± 0.22 | 0.774 | 2.96 ± 0.10 | 2.79 ± 0.26 | 0.803 | 4.67 ± 0.08 | 4.55 ± 0.18 | 0.689 | ||||||||||||||
22:5n6 | 0.24 ± 0.02 | 0.22 ± 0.13 | 0.775 | 0.75 ± 0.09 | 0.67 ± 0.12 | 0.813 | 0.46 ± 0.02 | 0.38 ± 0.12 | 0.457 | ||||||||||||||
22:5n3 | 0.20 ± 0.01 | 0.27 ± 0.02* | 0.546 | 0.3 ± 0.019 | 0.27 ± 0.02 | 0.803 | 0.46 ± 0.02 | 0.45 ± 0.03 | 0.814 | ||||||||||||||
22:6n3 | 1.75 ± 0.20 | 2 .00 ± 1.14 | 0.774 | 9.92 ± 0.33 | 10.55 ± 0.32 | 0.803 | 7.58 ± 0.32 | 7.03 ± 0.46 | 0.571 | ||||||||||||||
24:0 | 1.12 ± 0.11 | 1.04 ± 0.17 | 0.775 | 0.11 ± 0.01 | 0.1 ± 0.01 | 0.803 | 0.05 ± 0.01 | 0.19 ± 0.03** | 0.049 | ||||||||||||||
24:1n9 | 3.25 ± 0.14 | 3.27 ± 0.45 | 0.996 | 0.51 ± 0.08 | 0.5 ± 0.07 | 0.946 | 1.68 ± 0.19 | 1.4 ± 0.20 | 0.571 | ||||||||||||||
24:5n3 | 0.09 ± 0.00 | 0.11 ± 0.02 | 0.597 | 0.06 ± 0.00 | 0.05 ± 0.00 | 0.803 | 0.10 ± 0.01 | 0.12 ± 0.01 | 0.457 | ||||||||||||||
24:6n3 | 0.49 ± 0.03 | 0.61 ± 0.07* | 0.546 | 0.16 ± 0.02 | 0.13 ± 0.01 | 0.803 | 0.41 ± 0.019 | 0.36 ± 0.04 | 0.501 | ||||||||||||||
ACL | 18.39 ± 0.02 | 18.43 ± 0.06 | 0.775 | 18.31 ± 0.02 | 18.32 ± 0.03 | 0.9 | 18.53 ± 0.00 | 18.46 ± 0.01** | 0.049 | ||||||||||||||
SFA | 39.27 ± 0.35 | 38.03 ± 0.91 | 0.546 | 46.27 ± 0.66 | 46.95 ± 0.42 | 0.803 | 41.15 ± 0.24 | 42.45 ± 0.60* | 0.228 | ||||||||||||||
UFA | 60.73 ± 0.35 | 61.97 ± 0.91 | 0.546 | 53.73 ± 0.66 | 53.05 ± 0.42 | 0.803 | 58.85 ± 0.24 | 57.55 ± 0.60* | 0.228 | ||||||||||||||
MUFA | 46.61 ± 0.48 | 46.86 ± 2.88 | 0.874 | 29.16 ± 0.89 | 28.57 ± 1.17 | 0.813 | 35.36 ± 0.65 | 34.82 ± 1.06 | 0.738 | ||||||||||||||
PUFA | 14.12 ± 0.46 | 15.11 ± 2.03 | 0.597 | 24.57 ± 0.44 | 24.48 ± 0.90 | 0.944 | 23.49 ± 0.48 | 22.73 ± 0.60 | 0.571 | ||||||||||||||
PUFAn3 | 2.79 ± 0.21 | 3.24 ± 1.05 | 0.597 | 10.59 ± 0.33 | 11.12 ± 0.34 | 0.803 | 8.81 ± 0.33 | 8.2 ± 0.44 | 0.571 | ||||||||||||||
PUFAn6 | 11.33 ± 0.26 | 11.87 ± 0.98 | 0.597 | 13.98 ± 0.26 | 13.36 ± 0.61 | 0.803 | 14.68 ± 0.31 | 14.53 ± 0.39 | 0.805 | ||||||||||||||
DBI | 103.48 ± 2.01 | 108.63 ± 7.70 | 0.574 | 145.19 ± 1.90 | 145.55 ± 3.17 | 0.944 | 142.57 ± 1.87 | 137.95 ± 2.42 | 0.457 | ||||||||||||||
PI | 59.9 ± 2.74 | 65.76 ± 12.88 | 0.597 | 135.75 ± 3.00 | 137.92 ± 4.95 | 0.813 | 122.52 ± 3.02 | 117.35 ± 4.36 | 0.571 | ||||||||||||||
SFA/UFA | 0.65 ± 0.00 | 0.61 ± 0.02 | 0.546 | 0.86 ± 0.02 | 0.89 ± 0.01 | 0.803 | 0.7 ± 0.00 | 0.74 ± 0.01* | 0.228 | ||||||||||||||
PβOx | 3.69 ± 0.52 | 3.29 ± 7.47 | 0.775 | 74.66 ± 13.07 | 92.57 ± 14.00 | 0.803 | 19.17 ± 1.47 | 20.76 ± 6.38 | 0.716 | ||||||||||||||
FOREBRAIN | |||||||||||||||||||||||
DIENCEPHALON | SUBCORTICAL TELENCEPHALON | ||||||||||||||||||||||
Thalamus | Hippocampus | Caudate | Putamen | ||||||||||||||||||||
Middle-aged | Elderly | Sig | Middle-aged | Elderly | Sig | Middle-aged | Elderly | Sig | Middle-aged | Elderly | Sig | ||||||||||||
14:0 | 0.67 ± 0.04 | 0.68 ± 0.04 | 0.976 | 0.88 ± 0.01 | 0.79 ± 0.18 | 0.759 | 0.63 ± 0.02 | 0.6 ± 0.07 | 0.671 | 0.62 ± 0.01 | 0.64 ± 0.05 | 0.809 | |||||||||||
16:0 | 18.08 ± 0.64 | 18.78 ± 0.91 | 0.934 | 20.99 ± 0.65 | 21.7 ± 0.65 | 0.759 | 21.46 ± 0.35 | 21.55 ± 0.50 | 0.932 | 19.39 ± 0.35 | 20.13 ± 0.57 | 0.568 | |||||||||||
16:1n7 | 1.34 ± 0.06 | 1.23 ± 0.14 | 0.761 | 1.26 ± 0.05 | 1.21 ± 0.22 | 0.759 | 0.92 ± 0.04 | 0.87 ± 0.05 | 0.673 | 0.89 ± 0.03 | 1.13 ± 0.11 | 0.309 | |||||||||||
18:0 | 23.36 ± 0.25 | 22.37 ± 0.59 | 0.687 | 22.01 ± 0.23 | 22.3 ± 0.39 | 0.759 | 25.41 ± 0.20 | 25.63 ± 0.98 | 0.693 | 24.05 ± 0.33 | 23.42 ± 0.37 | 0.470 | |||||||||||
18:1n9 | 23.06 ± 0.93 | 23.41 ± 1.55 | 0.937 | 20.88 ± 0.89 | 20.18 ± 1.21 | 0.759 | 17.43 ± 0.50 | 17.04 ± 0.64 | 0.762 | 18.49 ± 0.37 | 19.77 ± 0.98 | 0.470 | |||||||||||
18:1n7 | 4.51 ± 0.09 | 4.42 ± 0.30 | 0.934 | 4.69 ± 0.14 | 4.56 ± 0.28 | 0.759 | 4.69 ± 0.08 | 4.56 ± 0.11 | 0.682 | 4.48 ± 0.17 | 4.75 ± 0.12 | 0.520 | |||||||||||
18:2n6 | 0.66 ± 0.07 | 1.33 ± 0.59 | 0.687 | 0.64 ± 0.06 | 0.69 ± 0.05 | 0.759 | 1.03 ± 0.11 | 1.01 ± 0.10 | 0.942 | 1.08 ± 0.09 | 0.94 ± 0.21 | 0.632 | |||||||||||
18:3n3 | 0.09 ± 0.00 | 0.08 ± 0.00 | 0.761 | 0.06 ± 0.00 | 0.05 ± 0.01 | 0.759 | 0.07 ± 0.00 | 0.05 ± 0.00* | 0.195 | 0.07 ± 0.00 | 0.07 ± 0.01 | 0.899 | |||||||||||
18:4n3 | 0.03 ± 0.00 | 0.04 ± 0.00 | 0.687 | 0.02 ± 0.00 | 0.02 ± 0.00 | 0.759 | 0.05 ± 0.00 | 0.04 ± 0.00 | 0.297 | 0.02 ± 0.00 | 0.02 ± 0.00 | 0.892 | |||||||||||
20:0 | 0.2 ± 0.00 | 0.33 ± 0.11 | 0.687 | 0.15 ± 0.01 | 0.15 ± 0.02 | 0.862 | 0.17 ± 0.00 | 0.2 ± 0.00* | 0.195 | 0.17 ± 0.00 | 0.19 ± 0.01 | 0.394 | |||||||||||
20:1n9 | 1.74 ± 0.14 | 1.53 ± 0.25 | 0.796 | 1 ± 0.15 | 0.81 ± 0.39 | 0.759 | 0.85 ± 0.07 | 0.66 ± 0.10 | 0.297 | 1.03 ± 0.06 | 1.01 ± 0.14 | 0.934 | |||||||||||
20:2n6 | 0.36 ± 0.03 | 0.33 ± 0.04 | 0.687 | 0.45 ± 0.07 | 0.37 ± 0.08 | 0.759 | 0.17 ± 0.01 | 0.14 ± 0.02 | 0.368 | 0.22 ± 0.01 | 0.2 ± 0.02 | 0.763 | |||||||||||
20:3n6 | 0.9 ± 0.05 | 0.86 ± 0.08 | 0.796 | 1.25 ± 0.08 | 1.19 ± 0.07 | 0.759 | 1.03 ± 0.05 | 1.04 ± 0.07 | 0.935 | 1.03 ± 0.06 | 0.89 ± 0.06 | 0.394 | |||||||||||
20:4n6 | 7.76 ± 0.29 | 8.9 ± 1.03 | 0.687 | 8.82 ± 0.40 | 9.3 ± 0.70 | 0.759 | 9.23 ± 0.28 | 10.44 ± 0.42* | 0.195 | 9.33 ± 0.21 | 9.45 ± 0.34 | 0.899 | |||||||||||
20:5n3 | 0.11 ± 0.01 | 0.11 ± 0.02 | 0.934 | 0.04 ± 0.01 | 0.04 ± 0.00 | 0.817 | 0.07 ± 0.00 | 0.08 ± 0.01 | 0.912 | 0.07 ± 0.01 | 0.06 ± 0.03 | 0.899 | |||||||||||
22:0 | 0.11 ± 0.01 | 0.18 ± 0.08 | 0.687 | 0.1 ± 0.01 | 0.09 ± 0.02 | 0.759 | 0.08 ± 0.00 | 0.07 ± 0.00 | 0.389 | 0.1 ± 0.00 | 0.09 ± 0.02 | 0.711 | |||||||||||
22:1n9 | 0.94 ± 0.03 | 0.81 ± 0.16 | 0.937 | 0.84 ± 0.10 | 0.55 ± 0.10* | 0.705 | 1.32 ± 0.07 | 1.24 ± 0.12 | 0.671 | 1.02 ± 0.03 | 0.4 ± 0.25 | 0.579 | |||||||||||
22:4n6 | 5.12 ± 0.13 | 4.27 ± 0.26* | 0.401 | 5.34 ± 0.09 | 4.96 ± 0.26* | 0.705 | 4.72 ± 0.06 | 4.51 ± 0.11 | 0.297 | 5.38 ± 0.10 | 4.68 ± 0.27** | 0.118 | |||||||||||
22:5n6 | 0.84 ± 0.08 | 0.6 ± 0.13 | 0.586 | 1.15 ± 0.11 | 1.03 ± 0.11 | 0.759 | 0.81 ± 0.06 | 0.63 ± 0.07 | 0.265 | 0.81 ± 0.05 | 0.56 ± 0.07* | 0.202 | |||||||||||
22:5n3 | 0.34 ± 0.01 | 0.41 ± 0.04 | 0.586 | 0.3 ± 0.01 | 0.31 ± 0.02 | 0.759 | 0.28 ± 0.01 | 0.29 ± 0.02 | 0.673 | 0.31 ± 0.01 | 0.31 ± 0.01 | 0.987 | |||||||||||
22:6n3 | 7.69 ± 0.60 | 7.72 ± 0.96 | 0.937 | 7.64 ± 0.50 | 8.53 ± 0.93 | 0.759 | 8.52 ± 0.26 | 8.67 ± 0.34 | 0.839 | 10 ± 0.24 | 9.87 ± 0.57 | 0.899 | |||||||||||
24:0 | 0.34 ± 0.08 | 0.27 ± 0.05 | 0.796 | 0.03 ± 0.00 | 0.03 ± 0.09 | 0.759 | 0.17 ± 0.02 | 0.09 ± 0.01* | 0.195 | 0.02 ± 0.00 | 0.02 ± 0.03* | 0.309 | |||||||||||
24:1n9 | 1.39 ± 0.24 | 1.04 ± 0.23 | 0.694 | 1.14 ± 0.20 | 0.89 ± 0.20 | 0.759 | 0.69 ± 0.08 | 0.45 ± 0.08 | 0.27 | 1.14 ± 0.10 | 1.06 ± 0.20 | 0.899 | |||||||||||
24:5n3 | 0.05 ± 0.00 | 0.07 ± 0.00* | 0.511 | 0.04 ± 0.00 | 0.04 ± 0.00 | 0.890 | 0.06 ± 0.00 | 0.06 ± 0.00 | 0.666 | 0.04 ± 0.00 | 0.06 ± 0.00 | 0.311 | |||||||||||
24:6n3 | 0.32 ± 0.04 | 0.25 ± 0.03 | 0.687 | 0.26 ± 0.04 | 0.2 ± 0.02 | 0.759 | 0.14 ± 0.01 | 0.09 ± 0.01* | 0.195 | 0.26 ± 0.03 | 0.28 ± 0.05 | 0.899 | |||||||||||
ACL | 18.53 ± 0.01 | 18.47 ± 0.01* | 0.416 | 18.46 ± 0.01 | 18.44 ± 0.03 | 0.759 | 18.45 ± 0.00 | 18.44 ± 0.01 | 0.601 | 18.6 ± 0.01 | 18.51 ± 0.02** | 0.167 | |||||||||||
SFA | 42.75 ± 0.62 | 42.6 ± 1.26 | 0.937 | 44.17 ± 0.69 | 45.06 ± 0.71 | 0.759 | 47.91 ± 0.33 | 48.13 ± 0.50 | 0.839 | 44.35 ± 0.31 | 44.49 ± 0.91 | 0.913 | |||||||||||
UFA | 57.25 ± 0.62 | 57.4 ± 1.26 | 0.937 | 55.83 ± 0.69 | 54.94 ± 0.71 | 0.759 | 52.09 ± 0.33 | 51.87 ± 0.50 | 0.839 | 55.65 ± 0.31 | 55.51 ± 0.91 | 0.913 | |||||||||||
MUFA | 32.98 ± 1.34 | 32.44 ± 2.05 | 0.937 | 29.82 ± 1.30 | 28.21 ± 2.38 | 0.759 | 25.91 ± 0.71 | 24.82 ± 0.78 | 0.601 | 27.04 ± 0.55 | 28.12 ± 1.32 | 0.711 | |||||||||||
PUFA | 24.27 ± 0.75 | 24.96 ± 1.06 | 0.796 | 26.01 ± 0.66 | 26.73 ± 1.82 | 0.759 | 26.18 ± 0.48 | 27.05 ± 0.47 | 0.535 | 28.61 ± 0.29 | 27.39 ± 0.63 | 0.319 | |||||||||||
PUFAn3 | 8.64 ± 0.58 | 8.68 ± 0.90 | 0.937 | 8.36 ± 0.47 | 9.19 ± 0.90 | 0.759 | 9.2 ± 0.24 | 9.28 ± 0.35 | 0.912 | 10.77 ± 0.20 | 10.66 ± 0.50 | 0.899 | |||||||||||
PUFAn6 | 15.64 ± 0.28 | 16.28 ± 1.30 | 0.796 | 17.65 ± 0.35 | 17.54 ± 0.94 | 0.863 | 16.98 ± 0.30 | 17.78 ± 0.41 | 0.379 | 17.84 ± 0.30 | 16.73 ± 0.29** | 0.167 | |||||||||||
DBI | 144.4 ± 2.97 | 145.16 ± 3.27 | 0.956 | 147.71 ± 2.51 | 150.64 ± 7.02 | 0.759 | 145.67 ± 1.56 | 148.21 ± 1.78 | 0.601 | 159.55 ± 0.88 | 155.72 ± 2.94 | 0.319 | |||||||||||
PI | 127.6 ± 5.46 | 128.06 ± 6.90 | 0.976 | 133.56 ± 4.69 | 139.7 ± 11.29 | 0.759 | 136.66 ± 2.59 | 140.35 ± 2.68 | 0.625 | 152.54 ± 1.55 | 147.47 ± 4.76 | 0.470 | |||||||||||
SFA/UFA | 0.75 ± 0.01 | 0.74 ± 0.04 | 0.937 | 0.79 ± 0.02 | 0.82 ± 0.02 | 0.759 | 0.92 ± 0.01 | 0.93 ± 0.01 | 0.839 | 0.8 ± 0.01 | 0.8 ± 0.03 | 0.899 | |||||||||||
PβOx | 29.85 ± 3.86 | 33.91 ± 6.53 | 0.934 | 37.07 ± 6.16 | 48.54 ± 9.09 | 0.759 | 70.59 ± 11.74 | 109.16 ± 14.86 | 0.249 | 43.02 ± 5.80 | 48.88 ± 10.68 | 0.892 | |||||||||||
FOREBRAIN | |||||||||||||||||||||||
CORTICAL TELENCEPHALON | |||||||||||||||||||||||
Occipital cortex | Parietal cortex | Temporal cortex | |||||||||||||||||||||
Middle-aged | Elderly | Sig | Middle-aged | Elderly | Sig | Middle-aged | Elderly | Sig | |||||||||||||||
14:0 | 0.81 ± 0.03 | 0.7 ± 0.04 | 0.494 | 0.84 ± 0.04 | 0.7 ± 0.02* | 0.557 | 0.82 ± 0.01 | 0.85 ± 0.03 | 0.773 | ||||||||||||||
16:0 | 21.76 ± 0.36 | 21.18 ± 0.37 | 0.825 | 21.11 ± 0.57 | 20.56 ± 0.49 | 0.846 | 24.73 ± 0.36 | 24.11 ± 0.83 | 0.503 | ||||||||||||||
16:1n7 | 1.17 ± 0.06 | 1.02 ± 0.13 | 0.705 | 1.3 ± 0.07 | 1.14 ± 0.07 | 0.846 | 0.98 ± 0.05 | 0.94 ± 0.10 | 0.773 | ||||||||||||||
18:0 | 24.52 ± 0.26 | 23.74 ± 0.49 | 0.494 | 24.38 ± 0.39 | 24.28 ± 0.38 | 0.973 | 24.87 ± 0.18 | 25.09 ± 0.12 | 0.756 | ||||||||||||||
18:1n9 | 17.89 ± 0.58 | 18.54 ± 0.92 | 0.977 | 19.72 ± 0.81 | 19.85 ± 0.63 | 0.973 | 15.11 ± 0.42 | 16.28 ± 0.69 | 0.231 | ||||||||||||||
18:1n7 | 3.94 ± 0.11 | 4.00 ± 0.19 | 0.982 | 4.3 ± 0.10 | 4.3 ± 0.26 | 0.996 | 3.33 ± 0.06 | 3.51 ± 0.19 | 0.699 | ||||||||||||||
18:2n6 | 1.05 ± 0.12 | 1.15 ± 0.12 | 0.977 | 1.21 ± 0.11 | 1.21 ± 0.13 | 0.996 | 0.94 ± 0.10 | 1.05 ± 0.10 | 0.773 | ||||||||||||||
18:3n3 | 0.06 ± 0.00 | 0.06 ± 0.00 | 0.982 | 0.08 ± 0.00 | 0.07 ± 0.00 | 0.846 | 0.04 ± 0.00 | 0.04 ± 0.00 | 0.978 | ||||||||||||||
18:4n3 | 0.03 ± 0.00 | 0.04 ± 0.00 | 0.494 | 0.04 ± 0.00 | 0.04 ± 0.00 | 0.973 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.823 | ||||||||||||||
20:0 | 0.19 ± 0.00 | 0.21 ± 0.00 | 0.494 | 0.21 ± 0.00 | 0.2 ± 0.00 | 0.973 | 0.16 ± 0.00 | 0.18 ± 0.00* | 0.147 | ||||||||||||||
20:1n9 | 1.03 ± 0.09 | 1.02 ± 0.11 | 0.987 | 1.19 ± 0.16 | 1.04 ± 0.16 | 0.863 | 0.46 ± 0.05 | 0.52 ± 0.17 | 0.773 | ||||||||||||||
20:2n6 | 0.25 ± 0.02 | 0.21 ± 0.02 | 0.817 | 0.30 ± 0.03 | 0.25 ± 0.01 | 0.846 | 0.16 ± 0.01 | 0.15 ± 0.05 | 0.773 | ||||||||||||||
20:3n6 | 0.99 ± 0.05 | 0.97 ± 0.07 | 0.982 | 1.08 ± 0.054 | 1.02 ± 0.07 | 0.863 | 1.11 ± 0.07 | 1.02 ± 0.06 | 0.756 | ||||||||||||||
20:4n6 | 7.33 ± 0.23 | 7.38 ± 0.31 | 0.982 | 7.71 ± 0.32 | 8.02 ± 0.27 | 0.876 | 9.96 ± 0.31 | 9.1 ± 0.41 | 0.231 | ||||||||||||||
20:5n3 | 0.06 ± 0.012 | 0.06 ± 0.00 | 0.982 | 0.07 ± 0.00 | 0.06 ± 0.02 | 0.921 | 0.08 ± 0.01 | 0.07 ± 0.00 | 0.773 | ||||||||||||||
22:0 | 0.09 ± 0.00 | 0.10 ± 0.00 | 0.982 | 0.11 ± 0.01 | 0.11 ± 0.01 | 0.973 | 0.05 ± 0.00 | 0.06 ± 0.01 | 0.756 | ||||||||||||||
22:1n9 | 2.18 ± 0.10 | 2.28 ± 0.27 | 0.982 | 0.74 ± 0.04 | 0.48 ± 0.07 | 0.846 | 0.86 ± 0.39 | 1.07 ± 0.20 | 0.782 | ||||||||||||||
22:4n6 | 3.9 ± 0.13 | 3.51 ± 0.12 | 0.494 | 4.08 ± 0.13 | 3.88 ± 0.12 | 0.846 | 4.43 ± 0.11 | 4.04 ± 0.15* | 0.173 | ||||||||||||||
22:5n6 | 0.78 ± 0.05 | 0.65 ± 0.09 | 0.592 | 0.84 ± 0.09 | 0.72 ± 0.09 | 0.846 | 0.96 ± 0.08 | 0.66 ± 0.06* | 0.164 | ||||||||||||||
22:5n3 | 0.33 ± 0.01 | 0.37 ± 0.02 | 0.502 | 0.3 ± 0.01 | 0.34 ± 0.03 | 0.759 | 0.34 ± 0.02 | 0.36 ± 0.03 | 0.773 | ||||||||||||||
22:6n3 | 10.49 ± 0.29 | 11.69 ± 0.59 | 0.502 | 8.95 ± 0.43 | 10.37 ± 0.46 | 0.557 | 10.04 ± 0.27 | 10.4 ± 0.57 | 0.756 | ||||||||||||||
24:0 | 0.14 ± 0.019 | 0.13 ± 0.032 | 0.982 | 0.22 ± 0.04 | 0.2 ± 0.03 | 0.973 | 0.04 ± 0.00 | 0.06 ± 0.04 | 0.509 | ||||||||||||||
24:1n9 | 0.80 ± 0.10 | 0.80 ± 0.16 | 0.987 | 0.97 ± 0.22 | 0.83 ± 0.13 | 0.936 | 0.24 ± 0.06 | 0.28 ± 0.22 | 0.773 | ||||||||||||||
24:5n3 | 0.04 ± 0.00 | 0.03 ± 0.00 | 0.792 | 0.05 ± 0.00 | 0.07 ± 0.01 | 0.742 | 0.06 ± 0.00 | 0.04 ± 0.00* | 0.147 | ||||||||||||||
24:6n3 | 0.16 ± 0.01 | 0.17 ± 0.02 | 0.982 | 0.19 ± 0.02 | 0.26 ± 0.07 | 0.846 | 0.19 ± 0.02 | 0.09 ± 0.03** | 0.057 | ||||||||||||||
ACL | 18.49 ± 0.01 | 18.54 ± 0.01* | 0.494 | 18.42 ± 0.02 | 18.47 ± 0.01 | 0.557 | 18.39 ± 0.01 | 18.38 ± 0.02 | 0.773 | ||||||||||||||
SFA | 47.51 ± 0.47 | 46.05 ± 0.71 | 0.494 | 46.88 ± 0.76 | 46.05 ± 0.78 | 0.846 | 50.68 ± 0.46 | 50.35 ± 0.77 | 0.773 | ||||||||||||||
UFA | 52.49 ± 0.47 | 53.95 ± 0.71 | 0.494 | 53.12 ± 0.76 | 53.95 ± 0.78 | 0.846 | 49.32 ± 0.46 | 49.65 ± 0.77 | 0.773 | ||||||||||||||
MUFA | 27.02 ± 0.85 | 27.66 ± 1.38 | 0.982 | 28.22 ± 1.23 | 27.66 ± 1.17 | 0.973 | 20.98 ± 0.71 | 22.6 ± 1.53 | 0.254 | ||||||||||||||
PUFA | 25.48 ± 0.48 | 26.3 ± 0.85 | 0.825 | 24.91 ± 0.54 | 26.3 ± 0.43 | 0.646 | 28.34 ± 0.46 | 27.06 ± 0.86 | 0.231 | ||||||||||||||
PUFAn3 | 11.17 ± 0.29 | 12.43 ± 0.58 | 0.494 | 9.68 ± 0.41 | 11.19 ± 0.42* | 0.557 | 10.77 ± 0.31 | 11.03 ± 0.57 | 0.773 | ||||||||||||||
PUFAn6 | 14.3 ± 0.39 | 13.86 ± 0.30 | 0.793 | 15.22 ± 0.36 | 15.11 ± 0.30 | 0.973 | 17.57 ± 0.42 | 16.02 ± 0.33* | 0.147 | ||||||||||||||
DBI | 147.77 ± 1.58 | 153.92 ± 3.38 | 0.500 | 143.21 ± 1.98 | 151.26 ± 1.78* | 0.557 | 152.85 ± 1.72 | 149.47 ± 3.06 | 0.517 | ||||||||||||||
PI | 141.57 ± 2.60 | 149.4 ± 5.77 | 0.705 | 132.58 ± 3.79 | 144.17 ± 3.66 | 0.557 | 152.02 ± 2.57 | 147.29 ± 5.51 | 0.517 | ||||||||||||||
SFA/UFA | 0.91 ± 0.01 | 0.85 ± 0.024 | 0.494 | 0.89 ± 0.02 | 0.85 ± 0.02 | 0.846 | 1.03 ± 0.01 | 1.01 ± 0.02 | 0.773 | ||||||||||||||
PβOx | 84.36 ± 14.11 | 80.57 ± 17.42 | 0.982 | 58.46 ± 12.84 | 60.12 ± 13.10 | 0.973 | 61.01 ± 11.706 | 135.92 ± 22.40** | 0.057 | ||||||||||||||
FOREBRAIN | |||||||||||||||||||||||
CORTICAL TELENCEPHALON | |||||||||||||||||||||||
Entorhinal cortex | Frontal cortex | Cingulate cortex | |||||||||||||||||||||
Middle-aged | Elderly | Sig | Middle-aged | Elderly | Sig | Middle-aged | Elderly | Sig | |||||||||||||||
14:0 | 0.79 ± 0.03 | 0.81 ± 0.03 | 0.866 | 0.84 ± 0.04 | 0.77 ± 0.04 | 0.953 | 0.95 ± 0.05 | 0.87 ± 0.03 | 0.934 | ||||||||||||||
16:0 | 20.98 ± 0.66 | 20.03 ± 0.85 | 0.695 | 22.16 ± 0.51 | 22.09 ± 0.36 | 0.964 | 23.73 ± 0.93 | 22.52 ± 0.80 | 0.934 | ||||||||||||||
16:1n7 | 1.29 ± 0.07 | 1.52 ± 0.17 | 0.691 | 1.38 ± 0.08 | 1.28 ± 0.08 | 0.953 | 1.38 ± 0.12 | 1.52 ± 0.15 | 0.934 | ||||||||||||||
18:0 | 24.38 ± 0.27 | 23.82 ± 0.36 | 0.691 | 24.33 ± 0.26 | 24.28 ± 0.49 | 0.964 | 22.79 ± 0.41 | 22.49 ± 0.42 | 0.934 | ||||||||||||||
18:1n9 | 18.74 ± 0.95 | 20.86 ± 1.28 | 0.691 | 17.94 ± 0.84 | 18.03 ± 0.56 | 0.964 | 18.56 ± 1.18 | 19.53 ± 0.87 | 0.934 | ||||||||||||||
18:1n7 | 4.05 ± 0.16 | 4.11 ± 0.22 | 0.908 | 3.91 ± 0.11 | 3.85 ± 0.25 | 0.964 | 3.97 ± 0.11 | 4.14 ± 0.28 | 0.934 | ||||||||||||||
18:2n6 | 0.78 ± 0.08 | 0.83 ± 0.09 | 0.866 | 1.13 ± 0.07 | 1.12 ± 0.10 | 0.964 | 0.81 ± 0.10 | 0.86 ± 0.08 | 0.934 | ||||||||||||||
18:3n3 | 0.05 ± 0.00 | 0.06 ± 0.00 | 0.695 | 0.06 ± 0.00 | 0.05 ± 0.00 | 0.953 | 0.06 ± 0.00 | 0.07 ± 0.00 | 0.934 | ||||||||||||||
18:4n3 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.759 | 0.04 ± 0.00 | 0.03 ± 0.00 | 0.953 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.934 | ||||||||||||||
20:0 | 0.21 ± 0.00 | 0.23 ± 0.01 | 0.691 | 0.25 ± 0.08 | 0.19 ± 0.01 | 0.953 | 0.19 ± 0.00 | 0.22 ± 0.00** | 0.376 | ||||||||||||||
20:1n9 | 0.88 ± 0.15 | 1.06 ± 0.14 | 0.705 | 0.82 ± 0.10 | 0.76 ± 0.09 | 0.964 | 0.94 ± 0.23 | 1.07 ± 0.18 | 0.934 | ||||||||||||||
20:2n6 | 0.31 ± 0.04 | 0.32 ± 0.03 | 0.975 | 0.24 ± 0.02 | 0.21 ± 0.03 | 0.953 | 0.3 ± 0.04 | 0.28 ± 0.04 | 0.934 | ||||||||||||||
20:3n6 | 1.02 ± 0.07 | 0.97 ± 0.06 | 0.851 | 1.05 ± 0.04 | 0.99 ± 0.10 | 0.953 | 1 ± 0.05 | 0.98 ± 0.07 | 0.970 | ||||||||||||||
20:4n6 | 8.91 ± 0.37 | 8.17 ± 0.34 | 0.691 | 8.38 ± 0.30 | 8.56 ± 0.28 | 0.964 | 8.12 ± 0.30 | 7.75 ± 0.35 | 0.934 | ||||||||||||||
20:5n3 | 0.06 ± 0.00 | 0.06 ± 0.01 | 0.866 | 0.08 ± 0.01 | 0.05 ± 0.00 | 0.953 | 0.06 ± 0.01 | 0.05 ± 0.01 | 0.934 | ||||||||||||||
22:0 | 0.09 ± 0.01 | 0.12 ± 0.01 | 0.691 | 0.08 ± 0.00 | 0.08 ± 0.00 | 0.953 | 0.08 ± 0.01 | 0.09 ± 0.01 | 0.934 | ||||||||||||||
22:1n9 | 0.7 ± 0.04 | 0.62 ± 0.06 | 0.691 | 1.04 ± 0.12 | 0.96 ± 0.07 | 0.964 | 0.75 ± 0.07 | 0.72 ± 0.07 | 0.934 | ||||||||||||||
22:4n6 | 4.94 ± 0.10 | 4.75 ± 0.17 | 0.691 | 4.1 ± 0.11 | 3.91 ± 0.23 | 0.953 | 4.72 ± 0.14 | 4.48 ± 0.15 | 0.934 | ||||||||||||||
22:5n6 | 0.88 ± 0.05 | 0.68 ± 0.13 | 0.691 | 0.96 ± 0.10 | 0.73 ± 0.16 | 0.953 | 1.03 ± 0.12 | 0.78 ± 0.08 | 0.934 | ||||||||||||||
22:5n3 | 0.3 ± 0.01 | 0.31 ± 0.02 | 0.908 | 0.31 ± 0.01 | 0.34 ± 0.02 | 0.953 | 0.3 ± 0.02 | 0.32 ± 0.02 | 0.934 | ||||||||||||||
22:6n3 | 9.29 ± 0.44 | 8.72 ± 0.78 | 0.763 | 9.97 ± 0.49 | 10.87 ± 0.53 | 0.953 | 9.08 ± 0.62 | 9.84 ± 0.57 | 0.934 | ||||||||||||||
24:0 | 0.23 ± 0.053 | 0.34 ± 0.07 | 0.691 | 0.16 ± 0.03 | 0.14 ± 0.02 | 0.964 | 0.2 ± 0.08 | 0.22 ± 0.04 | 0.970 | ||||||||||||||
24:1n9 | 0.85 ± 0.19 | 1.32 ± 0.31 | 0.691 | 0.58 ± 0.17 | 0.54 ± 0.11 | 0.964 | 0.74 ± 0.29 | 0.93 ± 0.24 | 0.934 | ||||||||||||||
24:5n3 | 0.04 ± 0.00 | 0.05 ± 0.00 | 0.695 | 0.05 ± 0.00 | 0.04 ± 0.00 | 0.953 | 0.04 ± 0.00 | 0.04 ± 0.00 | 0.934 | ||||||||||||||
24:6n3 | 0.17 ± 0.03 | 0.23 ± 0.04 | 0.695 | 0.13 ± 0.02 | 0.12 ± 0.01 | 0.964 | 0.15 ± 0.04 | 0.18 ± 0.03 | 0.934 | ||||||||||||||
ACL | 18.48 ± 0.01 | 18.48 ± 0.01 | 0.975 | 18.43 ± 0.00 | 18.44 ± 0.01 | 0.953 | 18.38 ± 0.02 | 18.42 ± 0.01 | 0.934 | ||||||||||||||
SFA | 46.68 ± 0.82 | 45.36 ± 1.07 | 0.691 | 47.83 ± 0.60 | 47.56 ± 0.52 | 0.964 | 47.95 ± 1.10 | 46.41 ± 0.91 | 0.934 | ||||||||||||||
UFA | 53.32 ± 0.82 | 54.64 ± 1.07 | 0.691 | 52.17 ± 0.60 | 52.44 ± 0.52 | 0.964 | 52.05 ± 1.10 | 53.59 ± 0.91 | 0.934 | ||||||||||||||
MUFA | 26.52 ± 1.39 | 29.48 ± 2.00 | 0.691 | 25.68 ± 1.20 | 25.42 ± 0.86 | 0.964 | 26.34 ± 1.90 | 27.93 ± 1.58 | 0.934 | ||||||||||||||
PUFA | 26.8 ± 0.58 | 25.16 ± 0.95 | 0.691 | 26.49 ± 0.68 | 27.02 ± 0.41 | 0.953 | 25.71 ± 0.85 | 25.67 ± 0.69 | 0.970 | ||||||||||||||
PUFAn3 | 9.95 ± 0.41 | 9.45 ± 0.73 | 0.787 | 10.63 ± 0.47 | 11.5 ± 0.53 | 0.953 | 9.73 ± 0.59 | 10.53 ± 0.56 | 0.934 | ||||||||||||||
PUFAn6 | 16.85 ± 0.26 | 15.71 ± 0.33* | 0.691 | 15.86 ± 0.32 | 15.52 ± 0.63 | 0.953 | 15.98 ± 0.34 | 15.13 ± 0.46 | 0.934 | ||||||||||||||
DBI | 150.66 ± 2.00 | 145.79 ± 3.38 | 0.691 | 149.4 ± 2.67 | 152.96 ± 1.19 | 0.953 | 145.79 ± 2.83 | 148.5 ± 2.14 | 0.934 | ||||||||||||||
PI | 142.85 ± 4.21 | 133.85 ± 6.83 | 0.691 | 143.49 ± 4.71 | 148.97 ± 2.63 | 0.953 | 137.84 ± 5.74 | 140.32 ± 4.55 | 0.934 | ||||||||||||||
SFA/UFA | 0.88 ± 0.02 | 0.83 ± 0.03 | 0.691 | 0.92 ± 0.02 | 0.91 ± 0.01 | 0.964 | 0.93 ± 0.03 | 0.87 ± 0.03 | 0.934 | ||||||||||||||
PβOx | 79.71 ± 15.08 | 58.42 ± 20.69 | 0.732 | 91.97 ± 13.39 | 105.31 ± 16.42 | 0.953 | 84.06 ± 12.88 | 75.12 ± 18.50 | 0.934 |