Fig. 3.

Old blood induces aging-like transcriptomic changes by regulating genes expressed in endothelial cells and smooth muscle cells in the mouse aorta To determine the expression pattern of concordant genes in endothelial cells (ECs) and vascular smooth muscle cells (SMCs), single-cell RNA-seq data were obtained in ECs, and SMCs derived from the mouse aorta and published by Deng et al [82] were reanalyzed. Panel A–C: Identification of ECs and SMCs based on differentially expressed marker genes. Panel C depicts two-dimensional tSNE plots based on differentially expressed marker genes, colored by cluster. Panels A and B depict the expression of a canonical endothelial cell marker (Pecam1) and a canonical smooth muscle cell marker (Myh11), respectively. Relative expression values for each cell in each cluster identified in the two-dimensional tSNE plots are shown. Panel D: Bubble plot shows the relative expression of the concordant genes in mouse aorta ECs and SMCs. Bubble size is proportional to the percentage of cells expressing a gene, and color intensity are proportional to average scaled gene expression within a cluster. Note that old blood induces aging-like transcriptomic changes in young mouse aortas by regulating genes expressed both in endothelial cells and smooth muscle cells