TABLE 2.
Purification of xanthan lyase from 2 liters of culture fluid of P. alginolyticus XL-1 grown on xanthan
| Purification step | Total protein (mg) | Total Ua | Sp act (U mg−1 of protein) | Purification factor |
|---|---|---|---|---|
| Cell-free culture broth | 517 | 50.0 | 0.10 | 1 |
| (NH4)2SO4 precipitation (60% saturation) | 75 | 32.8 | 0.44 | 4.4 |
| Phenyl Sepharose HP chromatography plus overnight dialysis | 8.5 | 19.5 | 2.29 | 22.9 |
| Source 15Q chromatography | 4.2 | 11.0 | 2.62 | 26.2 |
a
One unit is defined as the amount of enzyme that produces 1 μmol of 4,5-ene-glucuronic acid residues per min. Activity was calculated from the increase of A235; ɛ235 was 8.0 cm2 μmol−1.