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. 2022 May 13;10:853003. doi: 10.3389/fcell.2022.853003

FIGURE 1.

FIGURE 1

Nuclear GSK-3β and KRasG12D promote pancreatic ductal cell expansion and IPMN development. (A) Schematic representation of KNGC (LSL-KRasG12D/Rosa26-LSL-nuclear GSK-3β/Pdx1-Cre) mouse model. Blue triangles indicate loxP sites. (B) Kaplan-Meier survival curve of the indicated genotypes. (C) Immunofluorescence (IF) staining of HA (red), amylase (green) and CK19 (purple) from pancreatic sections of indicated genotypes at 4 weeks age. Nuclei were counter-stained with Hoechst (blue). Shown are representative image from three different mice. (D) H&E-stained pancreatic sections from KNGC mice of 4-week, 4-month, and 8-month. Black boxes indicate magnified area. Bars = 200 μm. (E) Quantification of pancreatic duct size distribution and distribution of pancreatic duct size for KNGC mice were analyzed and expressed as mean ± SEM. n = 5. ∗p < 0.05 4-month versus 4-week KNGC mice. # p < 0.05 8-month versus 4-month KNGC mice.