FIGURE 2.

Two strategies exist for implementing CFPS: the PURE system and the extract-based system. In the PURE system (left), each essential factor associated with protein translation is purified separately from overexpressing cells, including aaRS, ribosomes, translation factors, tRNAs, etc. In addition, the corresponding template and reaction buffer components are added to construct a functional translation system. The extract-based system (right) is simple and rapid, since it relies on preparing the native cellular extract by lysing cells.