Skip to main content
. 2022 May 13;10:891808. doi: 10.3389/fbioe.2022.891808

FIGURE 5.

FIGURE 5

Different strategies for the production of individual tRNAs in vitro (A) Enzymatic synthesis. Three different patterns for tRNA transcription are based on the T7RNP transcription process, including the direct pattern, hammerhead pattern and RNase P pattern (B) Chemical synthesis. The production of tRNA is based on solid-phase chemical synthesis, with each cycle encompassing de-blocking, coupling, capping, and oxidation.