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. 2022 May 13;10:891808. doi: 10.3389/fbioe.2022.891808

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Copyright © 2022 Li, Tang and Qi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Selection of different tRNA sets for the decoding of sfGFP templates with reduced-codons (A) The native genetic code table of E. coli (B) Two reduced genetic code tables for the production of different versions of sfGFP (sfGFP_RED20 and sfGFP; with or without wobble, respectively, Unused codons are represented in gray, while frequently used codons are represented in black (C) Simplified schematic of sfGFP protein translation based on the tRNA-depleted S30 extract and PURE ΔtRNA system. This sfGFP mRNA clearly shows the one-to-one correspondence in the codon-tRNA-amino acid relationship.