Fig. 3.

Endocytosis and ROS scavenging effects of AFGd-LDH in vitro. (a) Intracellular trafficking of Cy5.5-labeled AFGd-LDH in PC12 cells. (b) TEM image of AFGd-LDH internalized in PC12 cells. (c–d) ROS images inside PC12 cells after treatment with different samples (20 μg/mL) after 6 h incubation. (e) The cell viability of PC12 cells incubated with AFGd-LDH (2.5, 5, 10, 20, 50 μg/mL) for 48 h. (f) The cell viability of PC12 cells incubated with H₂O₂ and various concentrations (10, 20, 50 μg/mL) of AFGd-LDH for 48 h. (g) FITC/PI staining to assess the improving of H₂O₂- caused apoptosis by AFGd-LDH (20 μg/mL). PC12 cells were treated with H₂O₂, ATO + H₂O₂, Gd-LDH + H₂O₂ and AFGd-LDH + H₂O₂ for 48 h.