Figure 2.

Theintralaminar and mediodorsal nuclei are major cerebellar postsynaptic targets in the limbic thalamus. (A) Schematic of experimental approach for disynaptic pathway tracing. (B1,B2) Example images of bilateral Cre expression in DCN. Red: immunofluorescence for NeuN neural marker; Green: anti-Cre immunoreactivity; Yellow: merge. (B3) Heatmap of Cre immunofluorescence in DCN, normalized to NeuN signal and averaged across experiments, as a function of distance (in mm) from bregma. (C1,C2) Example images of thalamic neurons conditionally expressing tdTomato (red) upon transneuronal transfer of Cre from cerebellar presynaptic axons. Green: NeuN immunofluorescence. (C3) Heatmap of the relative distribution of tdTomato+ neurons across thalamic nuclei, normalized to the total number of labeled neurons and averaged across experiments, as a function of distance from bregma. (C4–C7) Example registration of tdTomato+ neurons to the Allen mouse brain atlas. Numbers at the bottom denote distance (in mm) from bregma. Antero-posterior coordinates for each nucleus can be found in Table 1. Scale bars: 500 μm.