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. 2022 May 13;16:879634. doi: 10.3389/fnsys.2022.879634

Figure 4.

Figure 4

Thalamicneurons receiving cerebellar input form synapses in the basolateral amygdala and also target the nucleus accumbens and prelimbic cortex. (A) Schematic diagram of the experimental approach. Targets of tdTomato+ axons of thalamic neurons receiving cerebellar input were identified through imaging. (B) Mosaic confocal images of tdTomato+ axons along the anterior-posterior axis of the BLA. (C) High resolution airyscan confocal images of tdTomato+ axons in the BLA colocalizing with presynaptic (vGLUT2) (C1) and postsynaptic (PSD95) (C2) markers of excitatory synapses. Green: vGLUT2, gray: PSD95, yellow/white in (C3): overlay. (D) tdTomato+ axons in nucleus accumbens (D1,D2) and prelimbic cortex (D3,D4). Yellow squares in (B1,B3,B5,D1,D3) show zoom-in areas for (B2,B4,B6,D2,D4) images, respectively. Numbers at the bottom of images indicate the distance (in mm) from bregma. Scale bars: (B1,B3,B5,D1,D3): 200 μm; (B2,B4,B6,D2,D4): 50 μm; (C1–C3): 5 μm.