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. 2022 May 13;16:879634. doi: 10.3389/fnsys.2022.879634

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Copyright © 2022 Jung, Vlasov, D’Ambra, Parigi, Baya, Frez, Villalobos, Fernandez-Frentzel, Anguiano, Ideguchi, Antzoulatos and Fioravante.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Centromedial and parafascicular neurons project to the basolateral amygdala andreceive functional monosynaptic input from the cerebellum.(A) Scatterplot of % neurons receiving DCN input vs. % neurons projecting to BLA, for limbic thalamus nuclei. (B1–B4) Airyscan confocal images of DCN axons (red) and BLA-projecting neurons (green) in the centromedial (CM; B1) and parafascicular (PF; B3) thalamic nuclei. (B2,B4,B5) Zoomed-in areas in yellow squares from (B1,B3). Scale bars: (B1,B3): 500 μm; (B2,B4): 20 μm; (B5): 5 μm. (C) Schematic diagram of ex vivo optophysiology approach to test for monosynaptic connections between DCN and CM/PF thalamic n. (D1–D3) Average ChR2-evoked synaptic current (teal), overlaid onto single trial responses (gray), at baseline (D1); upon addition of the action potential blocker tetrodotoxin (TTX, 1 μm; D2); after further addition of the potassium channel blocker 4-aminopyridine (4AP, 100 μm; D3). (D4) Time course of the wash-in experiment for the same example cell. (D5) Summary of effects on amplitude (mean ± SEM) of ChR2-evoked synaptic responses for the indicated conditions. Bsln: baseline. (D6) Average (± SEM) onset latency of ChR2-evoked responses at baseline.