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. 2022 May 13;13:814019. doi: 10.3389/fimmu.2022.814019

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Copyright © 2022 Eikmans, van der Keur, Anholts, Drabbels, van Beelen, de Sousa Lopes and van der Hoorn

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of classical trophoblast markers in primary trophoblast cultures. (A) Messenger RNA expression of GATA3, TFAP2c, ELF3, and ELF5 (n = 6 primary cultures; n = 2 MSC cultures). (B) Approximately 750 microRNA were screened in primary CTB/EVT (n = 3), along with JAR, JEG-3, and two MSC lines. Out of these, 116 microRNAs were included for hierarchical clustering. Twenty-five C19 microRNA (green boxes) were typically expressed in trophoblasts. (C) Expression level of five typical C19 microRNAs (open bars) and average level of all C19 microRNAs per group (black bars). All graphs are represented by means with SD.