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. 2022 May 13;13:814019. doi: 10.3389/fimmu.2022.814019

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Copyright © 2022 Eikmans, van der Keur, Anholts, Drabbels, van Beelen, de Sousa Lopes and van der Hoorn

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of HLA-C in trophoblasts. (A) Messenger RNA expression of HLA-C according to the HLA-C alleles. RNA from CTB cultures (n = 7) was analyzed by quantitative PCR using primer sets that are specific for different HLA-C alleles. The vertical axes represent relative mRNA expression of HLA-C alleles, corrected for the average signal of two reference genes (GAPDH, ACTB). The graph shows means with SEM of two independent experiments per culture. (B) HLA-C mRNA expression was determined by quantitative PCR using generic primers that targeted all alleles (left panel). HLA-C was also studied by flow cytometry using antibodies against pan-HLA class I (right panel). (C) Messenger RNA expression of HLA-A, -B, -C, -DRB1, and -G (left panel) was studied in CTB and EVT cultures. RNA was analyzed by quantitative PCR using locus-specific primer sets. The graph shows means with SEM. Surface expression of individual HLA-A and HLA-B alleles was investigated by flow cytometry using human monoclonal antibodies on four blood cell samples and two trophoblast cultures (right panel). The graph displays medians with interquartile ranges.