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. 2022 May 13;13:814019. doi: 10.3389/fimmu.2022.814019

Table 1.

Overview of primers used for quantitative PCR assays in the study.

Target Forward sequence Reverse sequence
ACTB ACCACACCTTCTACAATGAG TAGCACAGCCTGGATAGC
CGA AACCCATTCTTCTCCCAGCC GTGGACTCTGAGGTGACGTT
ELF3 GGCCGATGACTTGGTACTGA CAGCTCCTCAAGGGCACAAT
ELF5 TAAATCGGAAGCCCTGGCAA ACTAACCTTCGGTCAACCCG
EPCAM GAATGGCAAAGTATGAGAAGGCTGA TCCCACGCACACACATTTGTAA
GAPDH ACCCACTCCTCCACCTTTGAC TCCACCACCCTGTTGCTGTAG
GATA3 CTCATTAAGCCCAAGCGAAG GCATTCCTCCTCCAGAGTGT
HLA-A generic TGGAGGAGGAAYAGCTCAGATA CAAGGCAGCTGTCTCACAC
HLA-B generic CCTAGCAGTTGTGGTCATC AGCCCTGGGCACGGTCG
HLA-C generic TGTCCTGGYTGTCCTAGCT TGTCTCAGGCTTTACAAGYGA
HLA-C*01 CACAGACTGACCGAGTGAG CCCCAGGTCGCAGCCAC
HLA-C*02/12 CCGCGGGTATGACCAGTC CTCCAGGTAGGCTCTCCA
HLA-C*02/17 CGAGTGAACCTGCGGAAA GAGCCACTCCACGCACTC
HLA-C*03 CACAGACTGACCGAGTGAG AGCGTCTCCTTCCCATTCTT
HLA-C*04/18 CGAGTGAACCTGCGGAAA GCCCCAGGTCGCAGCCAA
HLA-C*05 CGAGTGAACCTGCGGAAA CGCGCGCTGCAGCGTCTT
HLA-C*06 TACTACAACCAGAGCGAGGA GGTCGCAGCCATACATCCA
HLA-C*07 TACTACAACCAGAGCGAGGA ACGGGCCGCCTCCA
HLA-C*07:03 TACTACAACCAGAGCGAGGA GAGCCACTCCACGCACAG
HLA-C*08 ACGACACGCAGTTCGTGCA GCGCAGGTTCCGCAGGC
HLA-C*14 CCACTCCATGAGGTATTTCTC GGTCGCAGCCAAACATCCA
HLA-C*16 CCGCGGGTATGACCAGTC CCCTCCAGGTAGGCTCTCT
HLA-C*18 AGTCCGAGAGGGGAGCCC GCCCCAGGTCGCAGCCAA
HLA-DRB1 generic ACAGTGGAATGGAGAGCACGG CCAGAGTGTCCTTTCTGATTCCT
HLA-G generic GACAGCGACTCGGCGT GTGTTCCGTGTCTCCTCT
HLA-G1 ATGCTGCAGCGCGCGGACC TGGGCAGGGAAGACTGCTTCCA
HLA-G2 ACCAGAGCGAGGCCAACCC GGCAGGGAAGACTGCTTCCA
HLA-G3 AACCAGAGCGAGGCCAAGCAG AGCTCCCTCCTTTTCAATCTGAG
ITGA1 TCAATGACTTTCAGCGGCCC ACCTCTCCCAACTGGACACT
ITGA5 GGCTTCAACTTAGACGCGGA GGCCGGTAAAACTCCACTGA
ITGA6 GAGCTTTTGTGATGGGCGATT CTCTCCACCAACTTCATAAGGC
TEAD4 TGGACAAGCCCATCGACAAT TGCCTGGTCCTTTAGCTTGG
TFAP2C TGGACGAGGTGCAGAATGTC TCAGTGGGGTTCATTACGGC