Figure 2.

Characterization of exosomes derived from RPTECs

(A) 2D STORM imaging of anti-CD9-labeled (Alexa 647 nm) exosomes from non-stimulated RPTECs (CK−) versus cytokine-stimulated RPTECs (CK+) (see section “materials and methods”). Photos are representative images. Scale bar, 1,000 nm. (B) Transmission electron microscopy (TEM) of exosomes derived from CK− versus CK+ RPTECs; arrows point to the exosome membrane bilayer. Scale bar, 50 nm. C) Time course analysis of exosomal release from CK− and CK+ RPTECs using Alexa (647 nm)-labeled CD9 antibody for exosome immunolabeling (see section “materials and methods”). Statistical analysis was performed using two-way ANOVA with multiple comparison test and Bonferroni correction (GraphPad Prism 8.0.1). Results are presented as mean ± SEM of two independent experiments. ∗∗p < 0.01. (D) Immunoblot analysis of TSG101 in exosome lysates of either CK− or CK+ sample. The image is a representative of three independent experiments.