Figure 7.

Cytokine-mediated release of candidate exosomal miRNAs and mt-tRNAs in RPTECs

(A) Absolute quantification PCR analysis of dysregulated exosomal miRNAs, hsa-miR-21-5p, -192-5p, and -215-5p, derived either from non-stimulated (CK−) or cytokine-stimulated (CK+) RPTECs. The percentage release of each candidate exosomal miRNA was calculated by obtaining the total RNA copies in exosomes and normalized to the total RNA copies in cells (set to 100%) (see section “materials and methods”). Data represent mean ± SD, which consists of three independent samples pooled together. (B) The percentage release of candidate exosomal mt-tRNAs, mt-tRNA^His, mt-tRNA^Leu1, and mt-tRNA^Val was analyzed similar to (A). Bar graph represents mean ± SEM of two independent experiments.