Figure 8.

Regulation of candidate exosomal miRNAs and mt-tRNAs from RPTECs by combinatorial treatment of GW4869 and manumycin A

(A–C) RT-qPCR analysis of dysregulated exosomal miRNAs, hsa-miR-21-5p, -215-5p, and -192-5p, derived from non-stimulated (CK−) or cytokine-stimulated (CK+) RPTECs treated with both inhibitors (2× inhib.), GW4869 (5 μM), and manumycin A (125 nM). The percentage release of each candidate exosomal miRNA was calculated by obtaining the total RNA copies in exosomes and normalized to the total RNA copies in cells (set to 100%) for each treatment (see section “materials and methods”). Data represent mean ± SD, which consists of three independent samples pooled together. (D–F) The percentage release of each candidate exosomal mt-tRNAs, mt-tRNA^His, mt-tRNA^Leu1, and mt-tRNA^Val was analyzed similar to (A–C). Data represent mean ± SEM of two independent experiments.