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Validation of reference gene selection. Relative expression levels of the target gene let-7-5p under different treatments. (A) Insecticide tolerance, (B) developmental stages, (C) adult tissues, and (D) genders. The results are displayed as the mean ± based on three independent biological replicates. The t-test was used for statistical difference analysis between the best combination of reference genes and the worst, and statistical differences were denoted by * (p < 0.05).
