TABLE 1.

Sequences and amplification characteristics of candidate reference genes.

Candidate	Primer sequence (5′→ 3′)	E (%) a	R 2 b	Slope
miR-1-3p	F: CGC​GTG​GAA​TGT​AAA​GAA​GTA​TGG​AG	98.27	0.9883	−3.364
miR-9a	F: CGC​TCT​TTG​GTA​TTC​TAG​CTG​TAG​GAT	94.51	0.9732	−3.461
miR-11	F: CGC​ATC​ACA​GTC​AGA​GTT​CTA​GCT	108.20	0.9944	−3.144
miR-100-5p	F: AAC​CCG​TAG​ATC​CGA​ACT​TGT​GAA	107.95	0.9930	−3.145
miR-184	F: CGT​GGA​CGG​AGA​ACT​GAT​AAG​GG	97.67	0.9988	−3.379
miR-252a	F: CTA​AGT​ACT​CCG​TGC​CGC​AGG​A	94.54	0.9630	−3.464
miR-275	F: TCA​GGT​ACC​TGA​AGT​AGC​GCG	95.68	0.9598	−3.433
miR-277	F: CGC​TAA​ATG​CAC​TAT​CTG​GTA​CGA​C	103.72	0.9905	−3.236
miR-279 d	F: CGC​GTG​ACT​AGA​TTT​TCA​CTC​ATT​C	107.81	0.9815	−3.148
miR-624	F: CGC​TAT​TCA​CCA​GTA​CTT​GTA​GTC​TCA	103.09	0.9564	−3.251
let-7-5p	F: CCG​CTG​AGG​TAG​TAG​GTT​GTA​TAG​T	113.59	0.9666	−3.034
U6 snRNA	F: Supplied with kit (TaKaRa catalog no. 638313)	97.51	0.9976	−3.384
	R: Supplied with kit (TaKaRa catalog no. 638313)
mRQ 3′ primer	Supplied with kit (TaKaRa catalog no. 638313)

^a E, amplification efficiency.

^b R ² , determination coefficient.