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. 2022 Apr 20;298(6):101958. doi: 10.1016/j.jbc.2022.101958

Figure 2.

Figure 2

Onset of photoreceptor apoptosis in Lpcat1 knockout (KO) mice.A, the average value of TUNEL-positive cells per outer nuclear layer (ONL) areas in the retinal section of postnatal day 6 (P6), P8, P11, P13, and P19 Lpcat1 heterozygous (HZ) and KO (n = 3 for each group). B, the ONL thickness in P6 and P8 Lpcat1 HZ and littermate KO mice. The left images showed the retinal structure stained with hematoxylin and eosin. Representative images from over three independent experiments were shown. The right bar graph showed the ONL thickness (P6, n = 3 for each group; P8, n = 6 for each group). Scale bars are 50 μm. C, the levels of rhodopsin mRNA in P6 and P8 of Lpcat1 HZ and littermate KO retina. The expression level was normalized by glyceraldehyde 3-phosphate dehydrogenase (Gapdh) (n = 3 for HZ, n = 4 for KO). D, the average value of TUNEL-positive cells per outer nuclear layer (ONL) areas in P9 dark-reared Lpcat1 HZ and littermate KO retina (n = 4 for HZ, n = 5 for KO). A–C, significance is based on two-way ANOVA followed by Bonferroni’s multiple comparisons test (∗p < 0.05, ∗∗p < 0.01, ns; no significance). Data are shown as mean + SD of independent experiments. D, significance is based on the unpaired t test (∗∗p < 0.01). Data are shown as mean + SD of independent experiments. TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling.