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. 2022 Apr 20;298(6):101958. doi: 10.1016/j.jbc.2022.101958

Figure 5.

Figure 5

Effect of LPCAT1 deficiency on the structure and function of photoreceptor cells.A, representative transmission electron microscopy (TEM) images of photoreceptor outer segment (OS) in 4-week-old Lpcat1 knockout (KO) and littermate wildtype (WT) mice (n = 3, for each group). Representative images from three independent experiments were shown. Scale bars are 1 μm. B, fatty acid composition of phosphatidylcholine (PC) in the isolated photoreceptor OS. OS samples were prepared from postnatal day 19 (P19) Lpcat1 heterozygous (HZ) and KO mice (n = 3 for each group). Significance is based on two-way ANOVA followed by Bonferroni’s multiple comparisons test (∗∗p < 0.01). Data are shown as mean + SD of independent experiments. C, representative images of PDE6β and rhodopsin in P13 Lpcat1 HZ and littermate KO mice by immunohistochemistry analysis. Signals were detected by 3,3′-diaminobenzidine (DAB, shown in brown), observed in the OS region (shown in red brackets). The dark brown colors in RPE and choroid were DAB-independent (n = 3 for each group). Representative images from three independent experiments were shown. Scale bars are 50 μm. D, cGMP level in P11 Lpcat1 HZ and littermate KO retina (n = 3 for HZ, n = 5 for KO). Data are shown as mean + SD of independent experiments. Significance is based on unpaired t test (∗p < 0.05). IS, inner segment; ONL, outer nuclear layer; OS, outer segment; RPE, retinal pigment epithelium.