Figure 1.

Construction of an infectious cDNA clone of HP-PRRSV TA-12 strain and the reporter viruses expressing a Gluc. (A) An infectious cDNA clone of TA-12 assembled through homologous recombination in vitro as described in the “Materials and Methods.” (B) Two cDNA clones were designed to express a Gluc via two strategies, respectively. In TA-Gluc1 construct the Gluc coding sequence followed by a PRRSV TRS6 was inserted into pCMV-TA-12 M between ORF1b and ORF2a, while in TA-Gluc2 construct a PRRSV TRS6 followed by the Gluc coding sequence was inserted between ORF7 and 3′UTR.