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. 2022 May 13;13:907281. doi: 10.3389/fmicb.2022.907281

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Copyright © 2022 Li, Ren, Li, Xiao and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The genetic stability of the Gluc-expressing reporter virus. The Gluc expression cassettes in the recombinant viruses of P5 and P10 were checked by RT-PCR (A) and DNA sequencing (B), whereas the corresponding regions in the parental viruses were amplified as a control. In addition, the Gluc expression levels by the recombinant viruses of P3, P5, and P10 were evaluated using the multiple-step growth curves (C). MARC-145 cells were infected with the recombinant viruses at an MOI of 0.01, culture supernatants were harvested at 12, 24, 36, 48, 60, and 72 hpi and titrated by luciferase assay. Each data point represents the mean ± deviation of triplicates.