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Serum neutralization assay using the Gluc reporter virus. (A) A schematic diagram of the procedure for serum neutralization assay. (B) The titers of neutralizing antibodies in pig serum samples were calculated based on the reduction of gaussia luciferase activity. Each data point represents the mean ± deviation of duplicates. (C) The neutralization of virus infection by selected serum samples was confirmed by IFA detection of N protein expression.
