Table 1.
The primers used in this study.
| Name | Sequence | Usage |
|---|---|---|
| F1-F | AGGTCTATATAAGCAGAGCTACGCGTTAATAGCATGACGTATAGGTGTTGGC | Construction of the full-length cDNA clone |
| F1-R | TGCGTAGCAGGATCACTAAG | |
| F2-F | GCTTGTGATGCGTCCAAG | |
| F2-R | TAATTGAATAGGAATTCGATATCCTTAAGTTCATTACCACCTGTAACG | |
| F3-F | CGTTACAGGTGGTAATGAACTTAA | |
| F3-R | ATGGCCAAAAATATGATGATATCAAC | |
| F4-F | TGGTGTCCATTGTTGATATCAT | |
| F4-R | ACTTTACCCCCACACGGT | |
| TA-12-EcoRV-F | GAATTTCTGGTGTCCATTGTTG | Introduction of a genetic marker into cDNA clone |
| TA-12-dBsrGI-R | GGAGGAATGGACAGCTATTAGCC | |
| TA-12-dBsrGI-F | GGCTAATAGCTGTCCATTCCTCCATA | |
| TA-12-BsrGI-R | ACATTGGCAGTGATGGTG | |
| TA-12-BsrGI-F | CACGGCGATAGGGACGCC | Construction of pCMV-TA-Gluc2 |
| TA-12-N/TRS6-R | AACTCTGGTTAAAGGGGTTGCCGCGGAACTCATGCTGAGGGTGATGCTGT | |
| TA-12-TRS6/Gluc-F | CAACCCCTTTAACCAGAGTTTCAGCGGAACAATGGGAGTCAAAGTTCTGTTTGCC | |
| TA-12-Gluc/UTR-R | TGCCAGCCCATTAGTCACCACCGGCCCC | |
| TA-12-Gluc/UTR-F | TGGTGACTAATGGGCTGGCATTCTTTGGC | |
| TA-12-NotI-R | CCGGAAGGGCTTAAAGCGG | |
| TA-12-BsaBI-F | CAAACACACCTGGGGATTT | Construction of pCMV-TA-Gluc1 |
| TA-12-nsp12/Gluc-R | TGACTCCCATTTCAATTCAGGCCTAAAGTTGG | |
| TA-12-nsp12/Gluc-F | CTGAATTGAAATGGGAGTCAAAGTTCTGTTTG | |
| TA-12-Gluc/TRS6-R | AACTCTGGTTAAAGGGGTTGCCGCGGAACTTAATTAATTAGTCACCACCGGCCCC | |
| TA-12-TRS6/GP2-F | CAACCCCTTTAACCAGAGTTTCAGCGGAACAATGAAATGGGGTCTATGCAAAGCC | |
| PRRSV-F1 | GTCAATCAGCTGTGCCA | RT-PCR and DNA sequencing |
| PRRSV-R | CCCTAATTGAATAGGTGRCTT |