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. 2022 May 13;13:854764. doi: 10.3389/fgene.2022.854764

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Copyright © 2022 Najar, Arefian, Sidransky, Gowda, Prasad, Modi and Chatterjee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detailed experimental workflow for phospho-(Y)-proteomic analysis, data analysis, and functional validation in gastric cancer cells between STO-609-treated and DMSO control conditions. (A) Schematic representation adapted for sample preparation for identification and quantification of phospho-(Y)-proteomic changes in gastric cancer cells (AGS) treated with STO-609 and vehicle control (DMSO). (B) Schematic representation LC-MS/MS analysis. (C) Schematic representation for PTM/protein identification and quantification from LC-MS/MS data using proteomic platform Proteome Discoverer. (D) Bioinformatics analysis/data analysis of proteomic data obtained in gastric cancer cells (AGS) treated with STO-609 and vehicle control (DMSO). (E) Validation of protein expression identified from proteomic data analysis.