Figure 5.

U1A upregulates several oncogenes and promotes cell migration

(A) mRNA expression levels of the genes indicated in the top of the figure were quantified by RT-qPCR in JHH6 cells transfected with control or siRNAs targeting U1A or U170K. RPLP0 mRNA levels were used as a reference for normalization. (B–D) Cells transfected as described in (A) were used to evaluate cell migration by evaluation of (B) FN1 and CDH2 mRNA levels by RT-qPCR using RPLP0 mRNA as a reference, (C) measuring human CTGF secretion by ELISA, or (D) evaluating cell migration in Transwell assays. Representative images were taken at 20× under bright-field conditions and the cell-stained area was quantified with FIJI image analysis software. The experiments were performed at least three times in duplicate. Error bars show SEM. Either two-tailed Student’s t test or one-way ANOVA were employed to compare two or more independent groups respectively. Results are indicated as ns, non-significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; and ∗∗∗∗p < 0.0001.