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. 2022 May 13;10:870745. doi: 10.3389/fcell.2022.870745

FIGURE 2.

FIGURE 2

Crystal structure of Mad1CTD-Bub1CD1 shows multiple direct interactions. (A) Crystal structure of Mad1CTD dimer (green/light blue) bound to two Bub1CD1 molecules (purple/yellow) from PDB: 7B1F (Fischer et al., 2021). (B) Close-up view of the interaction between Mad1 R617 with Bub1 pT461. The dark blue dashes indicate the hydrogen bonding. (C) Top view of the hydrophobic pocket formed by Mad1 L618 and Bub1 F470 and I471. (D) Close-up view of the interactions between Mad1 R650 and Bub1 Q476, Mad1 I643 and Bub1 F475, Mad1 F629 and Bub1 F475, Mad1 Q627 and Bub1 M474. The dark blue dashes indicate hydrogen bonding and the yellow dashes indicate π–π stacking. Pymol was used to visualize the structure and interactions between residues.