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. 2022 May 13;10:833579. doi: 10.3389/fcell.2022.833579

FIGURE 7.

FIGURE 7

LUCAT1 promotes glycolysis in LUAD cells through the LUCAT1/miR-4316/VEGFA axis. (A,B) Migration and invasion ability of LUAD cells were analyzed under normal culture conditions with high concentration of glucose; glycolysis inhibitor 2-DG abolished the ability of the migration and invasion ability of LUAD cells. (C,D) qRT-PCR was performed to detect the expression of Glycolysis-related enzyme markers in A549 cells after transfected with si-LUCAT1, pcDNA3.1- LUCAT1 or corresponding negative control. (E,F) Lactate production and glucose consumption level were measured after transfected with si-LUCAT1, pcDNA3.1- LUCAT1 or corresponding negative control. (G,H) Lactate production and glucose consumption level were measured after co-transfection with negative control mimics, miR-4316 mimics, or/and overexpression plasmid (pcDNA3.1- LUCAT1), using reagent kit according to the instruction of the manufacturers, respectively. (I,J) Lactate production and glucose consumption level were measured after co-transfection with negative control siRNA, si-LUCAT1, or/and overexpression plasmid (pcDNA3.1- LUCAT1), using reagent kit according to the instruction of the manufacturers, respectively. p-values were calculated by two-tailed student’s t-test or one-way ANOVA (*p < 0.05; **p < 0.01; ***p < 0.001).