Figure 3.

Reactants, intermediates, and final products observed from the reaction with persulfides

(A) Representation of the different species identified in for the mass spectrometry data from SqrR reactivity experiment with glutathione persulfide, where the cysteine residues, C41 and C107, are boxed and the rest of the protein is depicted by a black line. The main species observed are reduced (black), di (blue), tri (green 1), and tetra (green 2).

(B) Representation of the different species identified in for the mass spectrometry data from CstR reactivity experiment with cysteine persulfide, where the cysteine residues, C30 and C59, are boxed and the first protomer is depicted by a black line and the second protomer, which forms the intermolecular crosslink, is depicted by a gray line. The main species observed are reduced (black), derivatized monomer (red), closed/open (purple), di/di (blue) and closed/closed (green).