Persulfide reactivity buffer

Reagent	Final concentration	Amount
sodium phosphate pH 7.4 (300 mM)	150 mM	25 mL
EDTA (0.5 M)	2 mM	200 μL
ddH2O	n/a	24.8 mL
Total	n/a	50 mL

Stored anaerobically at room temperature, (22°C–26°C).