FIGURE 2.

Optimization of CRISPR–Cas12a system in ERA/CRISPR–Cas12a fluorescence system. (A) The importance of ERA amplification for CRISPR system detection. ERA + CRISPR: detection results of the whole reaction system; CRISPR: sample without ERA amplification and directly tested with the CRISPR system. Error bars in panels represent the mean ± SD, where n = 3 replicates. The optimized fluorescence intensity of the system on reaction screening of gRNAs (B), gRNA concentration (C), LbCas12a concentration (D), and F-Q concentration (E,F), respectively.