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Optimization of ERA in ERA/CRISPR–Cas12a fluorescence system. (A,B) Optimization of ERA primer. Template concentration: 107 copies/μL (A), 105 copies/μL (B). (C,D) Optimization of ERA temperature. Template concentration: 107 copies/μL (C), 105 copies/μL (D). (E,F) Optimization of ERA time. Template concentration: 105 copies/μL (E), 102 copies/μL (F). Error bars in panels represent the mean ± SD, where n = 3 replicates.
