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. 2022 May 13;13:811768. doi: 10.3389/fmicb.2022.811768

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Copyright © 2022 Deng, Hu, Tang, Liang, Su, Jiang, Hu, Ying, Zhen, Xiao and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of F-B concentration and reaction time by the ERA/CRISPR–Cas12a dipstick system. (A) Concentration optimization of the dipstick probe (F-B). The relative intensities of the band are shown at the top, and the dipstick results for different F-B concentrations are shown below. (B) An optimal incubation time of the CRISPR–Cas12a system. The black horizontal dashed line in the gray scan indicates the cutoff value set based on T/C values of the negative control. Error bars in panels represent the mean ± SD, where n = 3 replicates.