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. 2022 May 13;13:811768. doi: 10.3389/fmicb.2022.811768

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Copyright © 2022 Deng, Hu, Tang, Liang, Su, Jiang, Hu, Ying, Zhen, Xiao and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the specificity of the ERA/CRISPR–Cas12a dual system. Specificity test of the ERA/CRISPR–Cas12a fluorescence system (A) and dipstick system (B) for targeting M. pneumoniae. SAU: Staphylococcus aureus; PAE: Pseudomonas aeruginosa; AB: Acinetobacter baumannii; KPN: Klebsiella pneumoniae; HIB: Haemophilus influenzae; Cps: Chlamydia psittaci; Mpi: Mycoplasma pirum; MPe: Mycoplasma penetrans; MH: Mycoplasma hominis. The black horizontal dashed line in the gray scan indicates the cutoff value set based on T/C values of the negative control. Error bars in panels represent the mean ± SD, where n = 3 replicates. ***P < 0.001.