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. 2022 May 13;13:811768. doi: 10.3389/fmicb.2022.811768

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Copyright © 2022 Deng, Hu, Tang, Liang, Su, Jiang, Hu, Ying, Zhen, Xiao and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of 92 clinical samples by the ERA/CRISPR–Cas12a dual system for M. pneumoniae. For each group of results, the detection results of the ERA/CRISPR–Cas12a fluorescence system are displayed at the top, the gray scan results of the strips are displayed in the middle, and the bottom is the interpretation results of the dual system. Background subtracted fluorescence was the fluorescence intensity of the experimental group against the blank control. Black horizontal dashed line indicates the threshold for a positive result. + : indicates a positive sample, −: indicates a negative sample. Error bars in panels represent the mean ± SD, where n = 3 replicates.