TABLE 3.

Primers used in this study.

Primer	Sequence (5′→3′)	Description	Source/Reference
27F	AGAGTTTGATCCTGGCTCAG	Universal primers amplifying 16S rRNA gene	Wilson et al., 1990
1492R	GGTTACCTTGTTACGACTT
dnfA-upF	CAAATCCTTTTAAGCCTGCG	Universal primers amplifying complete dnfA of Alcaligenes.	This study
dnfA-downR	ACTTTGACGYTTCCGGCCT
dnfA-F	CGCGGATCATGACWATCAAAAGCTACGAAAC	Universal former primer with BamHI restriction site targeting dnfA of Alcaligenes for clones.	This study
dnfA-R1	CCCAAGCTTTTGCAGCGCCTCCTGTTGTTCG	Reverse primer with HindIII restriction site targeting dnfA of A. faecalis subsp. faecalis CGMCC 1.3937 for cloning pET21a-WP_035272004.1.	This study
dnfA-R2	CCCAAGCTTTTGCAGCGCCTCCTGTTGCTCG	Reverse primer with HindIII restriction site targeting dnfA of A. ammonioxydans HO-1T and A. aquatilis CGMCC 1.9053, for cloning pET21a-WP_003803202.1 and pET21a-WP_094197465.1, respectively.	This study
dnfA-R3	CCCAAGCTTTTGCAGCGCCTCCTGCTGTTCG	Reverse primer with HindIII restriction site targeting dnfA of A. faecalis subsp. faecalis CGMCC 1.2006T and A. faecalis subsp. phenolicus DSM 16503T, for cloning pET21a-WP_042487153.1 and pET21a-WP_045930341.1, respectively.	This study