FIGURE 1.

Yun is required for ISC proliferation. (A) esg ⁺ cells (green, by esg > GFP) in Control intestines at 29°C for 7 days (white arrowheads). (B) The number of esg + cells (green) is significantly decreased in esg ^ts  > yun ^shRNA intestines at 29°C for 7 days compared to control (white arrowheads). (C) Quantification of the relative number of esg + cells in Control and esg ^ts  > yun ^shRNA intestines. Mean ± SD is shown. n = 15. ****p < 0.0001. (D) pH 3 staining (red) in Control intestines at 29°C for 7 days (white arrowheads). (E) pH 3 staining (red) in esg ^ts  > yun ^shRNA intestines at 29°C for 7 days. (F) Quantification of the number of pH 3 cells per gut in Control and esg ^ts  > yun ^shRNA intestines. Mean ± SD is shown. n = 20. ****p < 0.0001. (G) ISCs (green, by Dl > GFP) in Control intestines at 29°C for 7 days (white arrowheads). (H) Compared with Control, the number of ISCs (green) is significantly decreased in Dl ^ts  > yun ^shRNA intestines at 29°C for 7 days (white arrowheads). (I) Quantification of the relative number of ISCs in Control and Dl ^ts  > yun ^shRNA intestines. Mean ± SD is shown. n = 15. ****p < 0.0001. (J) MARCM clones of FRT Control (green) at room temperature for 6 days after clone induction (ACI). (K) The clone size of yun ^Δ9 is significantly decreased (ACI 6 days). (L) Quantification of the clone size in Control and yun ^Δ9 mutant. Mean ± SD is shown. n = 15. ****p < 0.0001. (M) esg + cells (green, by esg > GFP) in Control intestines at 29°C for 7 days (white arrowheads). Note that this panel is same as panel A for easy comparison. (N) The number of esg + cells (green) is significantly increased in esg ^ts  > flag‐yun intestines at 29°C for 7 days compared to control (white arrowheads). (O) Quantification of the relative number of esg + cells in Control and esg ^ts  > flag‐yun intestines. Mean ± SD is shown. n = 18. **p < 0.01. In all panels except graphs, GFP is in green and blue indicates DAPI staining of DNA. Scale bars: 20 μm