FIGURE 5.

The phosphorylation of the five serine sites in Yun is collectively required for ISC proliferation. (A) MARCM clones of FRT control (green, ACI 6 days), Dl and Pros are in red. (B) The clone size of yun ^Δ9 is significantly decreased (green, ACI 6 days). (C) Overexpressing Yun can rescue the clone size of yun ^Δ9 (green, ACI 6 days). (D) Expression of yun(5SA) mutant cannot rescue the proliferation defects observed in yun ^Δ9 (green, ACI 6d). (E) Expression of yun(5SD) mutant partially rescues the proliferation defects observed in yun ^Δ9 (green, ACI 6 days). (F) Quantification of the clone size of indicated genotypes. Mean ± SD is shown. n = 20. ^ns p >0.05; *p < 0.05. (G) MARCM clones of FRT control (red, ACI 6 days). (H) The clone size of yun ^Δ9 is significantly decreased (red, ACI 6 days). (I) Overexpressing yun can rescue the clone size of yun ^Δ9 (red, ACI 6 days). (J, L, N, P, and R) Expression of different single phospho‐dead point mutants of yun(1SA) cannot rescue the proliferation defects observed in yun ^Δ9 (red, ACI 6 days). (K, M, O, Q, and S) Expression of different single phospho‐mimic point mutants of yun(1SD) partially rescue the proliferation defects observed in yun ^Δ9 (red, ACI 6 days). (T) Quantification of the clone size of indicated genotypes. Mean ± SD is shown. n ≥ 20. ^ns p >0.05; ****p < 0.0001. In all panels except graphs, GFP is in green and blue indicates DAPI staining of DNA. Scale bars: 20 μm