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. 2022 Apr 11;55(5):e13227. doi: 10.1111/cpr.13227

FIGURE 3.

FIGURE 3

Pro‐resolving effect of the RvE1 and LXA4 combination after ChemR23 and/or ALX inhibition. (A) Expression of RelA mRNA detected by qPCR. (B) NAD+/NADH ratio measured by an NAD+/NADH assay kit. (C) SIRT1, (D) SIRT6 and (E) SIRT7 mRNA levels detected by qPCR. (F) Expression of p65 protein in both cytoplasm and nucleus detected by western blotting, normalized to β‐actin (in cytoplasm) or PCNA (in nucleus). (G) Phosphorylation and acetylation levels of p65 and expressions of SIRT1, SIRT6 and SIRT7 protein (normalized to β‐tubulin) detected by western blotting. (H) Representative double‐immunofluorescence labelling images of SIRT7 (green) and p‐p65 (red), and the nuclei were stained with DAPI (blue). (I) Representative double‐immunofluorescence labelling images of SIRT7 (green) and Ac‐p65 (red), and the nuclei were stained with DAPI (blue). (*p < 0.05 and **p < 0.01). DAPI, 4′,6‐diamidino‐2‐phenylindole; LXA4, lipoxin A4; NAD+, nicotinamide adenine dinucleotide; qPCR, quantitative polymerase chain reaction; RvE1, resolvin E1