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. 2022 Apr 11;55(5):e13227. doi: 10.1111/cpr.13227

FIGURE 4.

FIGURE 4

Pro‐resolving effect of the RvE1 and LXA4 combination after SIRT7 silencing. (A) Silencing efficiency of si‐SIRT7 on mRNA level, and (B) that of in protein level. (C) Expression of RelA mRNA detected by qPCR. (D) NAD+/NADH ratio measured by an NAD+/NADH assay kit. (E) SIRT1 and (F) SIRT6 mRNA levels detected by qPCR. (G) Expression of p65 protein in both cytoplasm and nucleus detected by western blotting, normalized to β‐actin (in cytoplasm) or PCNA (in nucleus). (H) Phosphorylation and acetylation levels of p65 and expressions of SIRT1, SIRT6 and SIRT7 protein (normalized to β‐tubulin) detected by western blotting. (I) Representative double‐immunofluorescence labelling images of p‐p65 (green) and Ac‐p65 (red), and the nuclei were stained with DAPI (blue). (*p < 0.05 and **p < 0.01). DAPI, 4′,6‐diamidino‐2‐phenylindole; LXA4, lipoxin A4; NAD+, nicotinamide adenine dinucleotide; qPCR, quantitative polymerase chain reaction; RvE1, resolvin E1