TABLE 1.
Primers used for the selected human genes
| Target | Sequences (5′ → 3′) | Purpose |
|---|---|---|
| RelA | Forward: GGACATGGACTTCTCAGCCC | qPCR |
| Reverse: CACAAAGTTGGGGGCAGTTG | qPCR | |
| NLRP3 | Forward: GATCTTCGCTGCGATCAACAG | qPCR |
| Reverse: CGTGCATTATCTGAACCCCAC | qPCR | |
| caspase1 | Forward: TTTCCGCAAGGTTCGATTTTCA | qPCR |
| Reverse: GGCATCTGCGCTCTACCATC | qPCR | |
| IL‐1β | Forward: GGATCTCCTGTCCATCAGCC | qPCR |
| Reverse: GGAGCGAATGACAGAGGGTT | qPCR | |
| IL‐18 | Forward: TCTTCATTGACCAAGGAAATCGG | qPCR |
| Reverse: TCCGGGGTGCATTATCTCTAC | qPCR | |
| IL‐6 | Forward: TGCAATAACCACCCCTGACC | qPCR |
| Reverse: GTGCCCATGCTACATTTGCC | qPCR | |
| TNF‐α | Forward: CACAGTGAAGTGCTGGCAAC | qPCR |
| Reverse: AGGAAGGCCTAAGGTCCACT | qPCR | |
| CCL2 | Forward: TCAAACTGAAGCTCGCACTCT | qPCR |
| Reverse: GGGGCATTGATTGCATCTGG | qPCR | |
| CCL7 | Forward: AGAAGGACCACCAGTAGCCA | qPCR |
| Reverse: CCACTTCTGTGTGGGGTCAG | qPCR | |
| SIRT1 | Forward: TAGCCTTGTCAGATAAGGAAGGA | qPCR |
| Reverse: ACAGCTTCACAGTCAACTTTGT | qPCR | |
| SIRT6 | Forward: CAAGTGTAAGACGCAGTACGT | qPCR |
| Reverse: ATGTACCCAGCGTGATGGAC | qPCR | |
| SIRT7 | Forward: ATGAGCAGAAGCTGGTGC | qPCR |
| Reverse: CTGTCTGGTGTCTGTGGA | qPCR | |
| ALX | Forward: TCTTGCTCTAGTCCTTACCTTGC | qPCR |
| Reverse: AATGACAAACCGGATAATCCCTC | qPCR | |
| ChemR23 | Forward: TGGACTACCACTGGGTTTTCG | qPCR |
| Reverse: CGAGAGATGGGGAACTCAAGAAG | qPCR | |
| β‐Actin | Forward: CATGTACGTTGCTATCCAGGC | qPCR |
| Reverse: CTCCTTAATGTCACGCACGAT | qPCR | |
| si‐SIRT7 | CUCACCGUAUUUCUACUACUA | siRNA |
Abbreviation: qPCR, quantitative polymerase chain reaction.