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. 2022 May 27;18(5):e10441. doi: 10.15252/msb.202110441

Figure 1. Spontaneous cells with elevated SOS expression are more frequent in low nutrient conditions.

Figure 1

  1. Steady‐state distribution of GFP intensity from SOS reporter PsulA‐mGFP for WT cells in different growth conditions (blue M9‐gly, green M9‐glu, red M9‐glu+aa). GFP fluorescence intensity was measured as arbitrary units of fluorescence per unit cell area. Solid line represents the average frequency and shaded area the standard error from at least 3 replicates done on different days. Inset: A magnification of the second peak at high SOS expression.
  2. Steady‐state distribution of GFP intensity from SOS reporter PsulA‐mGFP for a strain unable to induce SOS (SOS‐off, lexA3 background) in different growth conditions. Solid line represents the average frequency and shaded area the standard error from at least 3 replicates done on different days. Inset: A magnification of the high‐fluorescence range of the distribution.
  3. Average GFP intensity for the lower 99% of the population of WT cells as a function of growth rate. Points represent the average and bars the standard error from at least 6 biological repeats.
  4. Average GFP intensity for the lower 99% of the population of lexA3 cells unable to induce SOS (SOS‐off, lexA3 background) as a function of growth rate. Stars represent the average and bars the standard error from at least 3 biological replicates done on different days.
  5. Average GFP intensity for the top 1% of the population of wild type cells as a function of growth rate. Points represent the average and bars the standard error from at least three biological replicates done on different days.
  6. Average GFP intensity for the top 1% of the population of lexA3 cells unable to induce SOS (SOS‐off, lexA3 background) as a function of growth rate. Stars represent the average and bars the standard error from at least three biological replicates done on different days.