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. 2021 Sep 25;12(5):2374–2390. doi: 10.1016/j.apsb.2021.09.020

Figure 1.

Figure 1

ISO induces hypertrophic responses accompanying with elevated miR-34c-5p expression. C57BL/6 mice were injected with ISO (2 mg/kg/day, 14 days). (A) Gross morphology of the hearts. (B) Representative images from echocardiography. (C)–(F) HE staining of cardiac sections, PSR staining for collagen deposition, WGA staining for cross-sectional areas. (G) and (H) HW/BW and HW/TL ratios. (I)–(N) Echocardiographic parameters. Data are shown as mean ± SD (n = 7); ∗P < 0.05, ∗∗P < 0.01 vs. control group. (O) and (P) Western blot and qRT-PCR were performed to measure the expression of ANF and β-MHC. (Q) The expression of miR-34c-5p was determined by qRT-PCR. Cultured NRCMs were treated with 10 μmol/L ISO for indicated time points. (R) and (S) Western blot was performed to measure the protein levels of ANF and β-MHC. The mRNA levels of Anf and β-Mhc were detected by qRT-PCR. (T) The level of miR-34c-5p was determined in NRCMs following ISO incubation. Data are shown as mean ± SD, n = 3 or 4; ∗P < 0.05, ∗∗P < 0.01 vs. control group.