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Further functionalization of glycoengineered trastuzumab via click chemistry. (A) Assembly of biotin, Cy3, and maltotriose onto glycoengineered trastuzumab. (B) LC‒MS of Az-LacNAc-Tras 6a. (C) LC‒MS of DBCO-LacNAc-Tras 6g. (D) LC‒MS of Biotin-LacNAc-Tras 9a. (E) LC‒MS of Cy3-LacNAc-Tras 9b. (F) Confocal imaging of cell-surface HER2 internalization using 9b.
