Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 24;12(5):2417–2428. doi: 10.1016/j.apsb.2021.12.013

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Synthesis of glycosite-specific ADCs using LacNAc-based substrates. (A) General procedure of one-step or two-step synthesis of gsADCs. (B) Small molecules used in gsADCs assembly. (C–E), LC‒MS profiles of Az-LacNAc-Tras 6a (C), gsADC-1 (D) and gsADC-2 (E). (F) SDS-PAGE of one-step synthesis of gsADC-2. Lane 0: protein marker; Lane 1: deglycosylated Tras (1a); Lane 2: gsADC-2. (G) Conjugation-site analysis of gsADC-2 via PNGase F digestion. (H) Antibody molecular weight of gsADC-2 after digestion with PNGase F. (I) HRMS profile of glycan–drug complex released from gsADC-2.