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. 2021 Nov 12;12(5):2592–2608. doi: 10.1016/j.apsb.2021.11.006

Figure 4.

Figure 4

In vitro activity of ZD-E-1. (A) H3cit protein expression and subcellular colocalization of 10 μmol/L ZD-E-1 and H3cit protein in 4T1 cells determined by CLSM (Leica). Scale bar = 50 μm. (B) Radial distribution of green and red fluorescence intensity analyzed by ImageJ. (C) Mean fluorescence intensity of H3cit calculated by ImageJ. (D and E) Effect of 10 μmol/L drug on ability of neutrophils to release NETs. Scale bar = 50 μm. (F and G) Representative images and analysis of transwell assay. a‒e was control, 10 μmol/L RGDS, 10 μmol/L ZD-E-1, 5 μmol/L ZD-E-1, and 2 μmol/L ZD-E-1, respectively. Scale bar = 100 μm. (H and I) Morphological changes in cells in the control and 10 μmol/L ZD-E-1 administration groups observed by TEM (Hitachi) and SEM (Hitachi). TEM scale bar = 1 and 0.5 μm. SEM scale bar = 20 and 10 μm. Data are presented as mean ± SD (n = 3). ∗P < 0.05 and ∗∗P < 0.01.