Figure 2.

DLE improved the histopathological changes in db/db mice. At the endpoint, the mice were sacrificed, and different tissues were sectioned for HE staining or ORO staining for histopathological observation. HE staining of (A) liver tissue, (C) pancreatic tissue, (D) kidney tissue, and (E) fat tissue, and (B) ORO staining of liver tissue are shown. The complete panel was presented in Fig. S2. (F) The mean adipocyte size of the normal group, model group, and DLE group. The sizes of ten randomly selected adipocytes stained by HE were quantified by ImageJ. The data are shown as the mean ± SD (n = 3). Statistical analyses were conducted using unpaired Student's t-tests. ∗∗P < 0.01 vs. the model group. HE, hematoxylin–eosin; ORO, oil red O.