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. 2021 Dec 29;12(5):2280–2299. doi: 10.1016/j.apsb.2021.12.014

Figure 3.

Figure 3

Epigenetic landscape of P300-BRD4 dependent inflammatory genes in Cur treated FCs. (A) ChIP analysis of the enrichment of P300, BRD2, BRD3, BRD4 and H3 at −1 kb from the promoter, at the promoter and at +1 kb from the promoter of inflammatory genes (IL-8, IL-1β) in ox-LDL-treated macrophages combined with JQ-1 or siBRD4. (B, C) ChIP analysis of the enrichment of P300, BRD2, BRD3, BRD4 and H3 at −1 kb from the promoter, at the promoter and at +1 kb from the promoter of inflammatory genes (IL-8, IL-1β) in ox-LDL-treated macrophages combined with Cur in the presence of oeBRD4 (B) or CTB (C). (D) Immunoblot analysis of the expressions of P300, BRD4, actin, acetyl-H3 and H3 in Cur-treated FCs in the presence of oeBRD4 and CTB. Data are expressed as mean ± SEM, n = 5. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; #P < 0.05, ###P < 0.001; ns, no significance.