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. 2021 Dec 29;12(5):2280–2299. doi: 10.1016/j.apsb.2021.12.014

Figure 7.

Figure 7

Specific ectopic expression of BRD4 and TFEB in macrophages reduces the protective effect of Cur in Apoe–/– mice. (A) Immunoblot analysis of BRD4 in aortas from Apoe–/– mice fed with the normal chow (NC) and high-fat diet (HFD). All of the Apoe–/– mice were fed with the HFD throughout the experiment. (B) Relative Brd4 mRNA level in Apoe–/– mice bone marrow cells (BMCs), which was transplanted with pLVCD68-Brd4 overexpression transduced BMCs combined with Cur. (C) Representative images of ORO-stained aortic lesion in Apoe–/– mice treated as in (B). (D) Representative images of ORO, hematoxylin and eosin, Sirius red and Masson's trichrome staining of cross-sections of the aorta from mice treated as in (B). Scale bar = 50 μm. (E) Relative mRNA levels of inflammatory genes of aortas from Apoe–/– mice treated as in (B). (F) Immunoblot analysis of TFEB in Apoe–/– mice fed with a HFD and treated with Cur. (G) Relative Tfeb mRNA level in Apoe–/– mice BMCs, which was transplanted with pLVCD68-Tfeb knock-out (TFEB KO) transduced BMCs combined with Cur. (H) Representative images of ORO-stained aortic lesion in Apoe–/– mice treated as in (G). (I) Representative images of ORO, hematoxylin and eosin, Sirius red and Masson's trichrome staining of cross-sections of the aorta from mice treated as in (G). Scale bar = 50 μm. (J) Relative mRNA levels of inflammatory genes of the aorta from mice treated as in (G). (K) Dihydroethidium (DHE) staining was used to detect the ROS content of aorta from mice treated as in (G). Scale bar = 20 μm. (L) Immunoblot analysis of LC3 II, P62, BRD4, and Acetyl-H3 in aorta from mice treated as in (G). Data are expressed as mean ± SEM, n = 5. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; ###P < 0.001.